The purposes of this study were (1) to acoustically compare three contrasting choral formations (block sectional, mixed, and sectional in columns) in performance of two contrasting pieces of choral literature (homophonic and polyphonic) by a university graduate choir (N=30), and (2) to compare singers¹ perceptions of and preferences for the contrasting choral formations across vocal part and gender. Both long-term average spectrums (LTAS) and perceptual chorister ratings were examined for differences. Acoustic results demonstrated no differences between the formations. However, LTAS differences attributable to the homophonic and polyphonic literature selections were observed. Chorister ratings indicated significant gender differences across many of the perception and preference items surveyed, particularly with respect to the sectional in columns formation.